Figure 8.

Human umbilical vein endothelial cell (HUVEC) protein expression of inducible nitric oxide synthase (iNOS) in response to high and/or low glucose exposure. High and/or low glucose exposure results in significant overexpression of iNOS protein expression in endothelial cells. HUVECs were incubated with each of the previously described glucose treatments at the indicated population doubling time points. (a) Western blot analyses of iNOS expression were performed with 20 μg of cell lysate. (b) Relative iNOS protein expression was quantified by densitometric analysis associated with Licor Odyssey software (data are normalized against GAPDH and expressed as means ± SE). Data are presented as means ± SE in four experiments. *p < 0.05, significantly different from control HUVECs cultured in the absence of high glucose.