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. 2013 Apr 12;68(12):1469–1481. doi: 10.1093/gerona/glt033

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© The Author 2013. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

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Figure 9. — Real-time quantitative reverse transcription PCR (qRT-PCR) quantification of gene expression in human umbilical vein endothelial cells (HUVECs) treated with high and/or low glucose. Determination by qRT-PCR of (a) eNOS mRNA (b) iNOS mRNA, and (c) AKT mRNA levels, in control (normal 5.5mM) and experimental HUVECs exposed to high (25mM) and/or low (1.5mM) glucose concentration at the designated population doubling time point. (d) The results represent the ratio generated by dividing the values of the above-mentioned amplified transcripts and constitutive GAPDH determined by optical density. *p < .05 of the experimental HUVECs in comparison with their respective control HUVECs. ◊ = NG, ■ = HG, X = Alt HG/NG, ▲ = Alt LG/NG, ● = Alt LG/HG.