Figure 7.
PEPCK activity in rat hepatocytes stimulated with MIF, dexamethasone, or MIF plus dexamethasone. Rat hepatoma H-4-II-E cells (2 × 106 cells per assay) were incubated for 6 hours with rMIF (0.1–10.0 ng/ml), or dexamethasone (10–8 M) given either alone or in combination, in which case cells were preincubated for 1 hour with rMIF before the addition of dexamethasone. Cytosolic PEPCK activity was measured as described in Methods and expressed as counts per minute per microgram of cytosolic protein. Data are a mean of two separate experiments.