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. 2013 Oct 31;9(10):e1003879. doi: 10.1371/journal.pgen.1003879

Figure 2. dCAP-D3 binds to loci containing retrotransposons.

Figure 2

ChIP performed for dCAP-D3 at the (A) mdg1-1403 locus or (B) G2-1077 locus demonstrate binding over the entire region with the peak of binding occurring at the region encompassing both retrotransposon sequence and neighboring DNA sequence. Black bars indicate ChIP signal from SG4 cells treated with control dsRNA for 4 days and white bars indicate ChIP signal from cells treated with dCAP-D3 dsRNA for 4 days. Primer sets used are depicted above the charts. Primer sets “LTR” and “5” (mdg1-1403 locus) and “4” (G2-1077 locus) are not specific for each of the loci but instead prime global retrotransposon sequence. Results are the averages of 2 experiments involving duplicate IPs and are presented as a percentage of the IP with control IgG ChIP signal subtracted. (*) indicates a quantitative comparison between dCAP-D3 signal in control dsRNA and dCAP-D3 dsRNA treated cells with a p-value less than 0.05 as calculated by student unpaired t-test. (+) indicates a quantitative comparison of specific dCAP-D3 signal to the average over the entire locus with a p-value less than 0.05 as calculated by student unpaired t-test.