Figure 4. Heat map representation of the expression levels of the pLS20cat genes at late exponential phase under various conditions analyzed by RNAseq.

Left lane (“wt”) shows the expression level of pLS20cat genes in the wild type strain background at late exponential phase when conjugation efficiency is at its maximum. Expression levels are presented on a log₂ scale covering a range from 0 (white, lowest level) to 16 (blue, highest level). Middle (+Rco) and right (+Rap) lanes represent the effects of ectopic expression of Rco_LS20 (middle lane) or Rap_LS20 (right lane), respectively, on the expression of the pLS20cat genes. Differential expression levels are presented on a log₂ scale covering a range of −16 to 16 using shades of red and green for repression and overexpression, respectively. White reflects no change in expression. Gene numbers according to our nomenclature and those deposited in database under accession number NC_015148.1 (preceded by “J”) are given on the right). “c” corresponds to leftward oriented genes.