Table 1. pLS20 gene 27c (rcoLS20) encodes a repressor of conjugation.
Strain | genotype | Plasmid | IPTG (1 mM) | Conjugation efficiency * |
PKS11 | 168 (wt) | pLS20cat | − | 5.6 10−3 |
+ | 3.8 10−3 | |||
PKS14 | 168, amyE::Pspank- rcoLS20 | pLS20cat | − | 1.6 10−4 |
+ | <1 10−8 | |||
PKS86 | 168, amyE::Pspank- rcoLS20 | pLS20rco | − | 5.7×10−2 |
+ | <1×10−8 |
: Conjugation efficiencies are calculated as transconjugants/donor. Conjugation efficiencies are the mean value of at least three independent experiments.