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. 2013 Oct 31;9(10):e1003692. doi: 10.1371/journal.ppat.1003692

Figure 4. Detection of OvPrPSc by sPMCA.

Figure 4

In A and E, the first two lanes of each panel correspond to brain extracts from Tg(OvPrP-A136)3533+/− and Tg(OvPrP-V136)4166+/− mice respectively without PK treatment. A136, V136, and A136/V136 refer to substrates from Tg(OvPrP-A136)3533+/− mice, Tg(OvPrP-V136)4166+/− mice, and mixtures of the two respectively. In B and F, the first lanes of each panel correspond to brain extracts from Tg(OvPrP-A136)3533+/− mice without PK treatment; in C and G, the first lanes of each panel correspond to brain extracts from Tg(OvPrP-V136)4166+/− mice without PK treatment; in D and H, the first lanes of each panel correspond to mixtures of brain extracts from Tg(OvPrP-A136)3533+/− and Tg(OvPrP-V136)4166+/− without PK treatment. In B–D and F–H, three different brains, indicated by #1–#3 were used as sources of substrates. In each case, the second lane of the second panel contains PK treated SSBP/1. Panels A and E are sPMCA using the indicated templates in the absence of a prion seed. Numbers in black above each lane refer to the round of sPMCA. C refers to a control in which no SSBP/1 seed was added. In each case C, and all numbered lanes were treated with PK. Blots were probed with mAbs 6H4 or PRC5 as indicated.