Table 1. CPIJ005623 KD effects in C. molestus Italy and C. quinquefasciatus Pel females in compatible crosses.
Time Point | Gene KD | Ind# | Total egg# | P [%] | p-valueφ | Mean embryo/Ind | [%] StI∶III | p-valueξ | |||
H | U | T | STI | STIII | |||||||
Compatible Cross: Italy KD females × Italy males | |||||||||||
T1 | iLacZ | 20 | 374 | 62 | 38 | 7.1 | 3.6 | 3.4 | 50∶50 | ||
iCPJI005623 | 20 | 396 | 51 | 49 | <0.0001 | 9.6 | 7.6 | 2 | 80∶20 | <0.0001 | |
Compatible Cross: Pel KD females × Pel males | |||||||||||
T1 | iLacZ | 19 | 1450 | 88 | 12 | 9 | 3 | 6 | 30∶70 | ||
iCPJI005623 | 19 | 1235 | 83 | 16 | 0.588 | 11 | 7 | 4 | 60∶40 | <0.0001 |
KD, Knockdown; Ind #, number of Individuals, P[%], percentage of hatched (H) and unhatched (U) embryos;
p-value, Welch's t-test on difference between hatched and unhatched embryos;
Mean embryo/ind, mean number of unhatched embryos undergoing CI and scored for developmental progression stage per individual; T = total; STI, stage I; STIII, stage II and III;
p-value on difference between stage I and stage II&III embryo proportions determined via GLM with binomial error structures.