Figure 7.

Schematic of siRNA trigger-mediated silencing in E. histolytica. (A) Functional sRNAs are generated to the reporter gene fused to the trigger and the construct is silenced by these sRNAs. sRNAs are lost after plasmid removal. (B) Functional sRNAs are generated to an amebic gene fused to the trigger, which silence the endogenous gene and exogenous constructs with sequence identity to the silenced gene. The sRNAs and gene silencing remain after plasmid removal. (C) No functional sRNAs are generated if a promoter does not drive the trigger fusion construct.