Figure 1.

Comparison of structure and function between long and short gRNAs. (A) Schematic illustrations of long and short gRNAs for Rosa26 target locus. Target sequence was underlined. (B) Genome modification efficiency of long and short gRNAs evaluated by T7EI assay. Genomic PCR amplicons of blastocysts derived from fertilized eggs injected with CAS9 mRNA (100 µg/ml), and long or short gRNA (10 µg/ml) were subjected for T7EI assay. Experiments were repeated for six times. An example of typical results of T7EI assay was shown in upper part. The arrow and arrowhead indicate PCR amplicons and digested fragments by T7EI, respectively. Calculated efficiencies were shown as boxplot in lower part.