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. 2000 Nov 15;106(10):1251–1261. doi: 10.1172/JCI10590

Figure 4.

Figure 4

Cytokine secretion by EBV-specific T cells during the primary immune response. PBMCs from HLA-A2+ individuals with IM (NS120 and NS122) were stained with the HLA-A2/GLCTLVAML tetrameric complex. PBMCs from NS120 were then cultured in vitro for 6 hours in the absence (a and b) or the presence (c and d) of 10 μM GLCTLVAML peptide in R10 supplemented with rIL-2. PBMCs from NS122 were cultured in vitro for 6 hours in the absence (e and f) or presence (g and h) of an autologous lymphoblastoid B-cell line (at a ratio of 1 B cell/1 PBMC) prepulsed with 10 μM GLCTLVAML peptide in R10 supplemented with rIL-2. Brefeldin A was added to the cultures after the first hour. Cells were surface-stained with an anti-CD8 mAb, fixed, permeabilized, and stained for intracellular IFN-γ (a, c, e, and g) or MIP1 β (b, d, f, and h). Only CD8+ T cells were included in the analyses. The frequency (%) of tetramer-reactive T cells that stained positively with mAb’s specific for IFN-γ or MIP1 β is shown.