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Effects of C5a blockade on cytochrome c release from mitochondria in thymocytes during CLP-induced sepsis. Cytosolic and mitochondrial fractions were separated as described in methods. (a and b) Cytochrome c concentrations in cytosol and mitochondria were quantitatively determined by ELISA. P < 0.05 when compared with sham or CLP + αC5a group. All values represent means ± SEM (n = 4).
