Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 5;41(20):e192. doi: 10.1093/nar/gkt797

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Luciferase expression in HEK 293 cells transfected with several RILES plasmids. Dose–response study of luciferase expression in HEK 293 cells transfected with (A) pRILES/siRNA tGFP T or (B) pRILES/122T in presence of (A) increasing amounts of siRNA tGFP (pU6/shRNA tGFP) and control siRNA (pU6/shRNA Ctl) or (B) increasing concentrations of synthetic miRNA-122. Selective luciferase expression in HEK 293 cells transfected either with (C) pRILES/122T, (D) pRILES/133T or (E) pRILES/221T in the presence of two concentrations of synthetic miRNA-122, −133 and −221. Forty-eight hours after transfection, luciferase expression in cells was determined and expressed as fold induction relative to control cells transfected with the plasmids alone and set to the arbitral value of 1. Data shown are the mean ±SD of one representative experiment performed in triplicate and reproduced at least three times. Statistics by the two-tailed t-test, *P < 0.05; **P < 0.01, n.s (no statistically significant difference) compared to control cells.