Table 1.

CRISPR on- and off-target cleavage rates

Guide strand	Mismatchesa	Indel % by sequencing (%)	Indel % by T7E1 (%)	b210987654321nGG	Region	Gene
R-01	0	67	54	GTGAACGTGGATGAAGTTGGtGG	Exon	HBB
	1	30	27	GTGAACGTGGATGcAGTTGGtGG	Exon	HBD
R-02	1	75	66	cTTGCCCCACAGGGCAGTAAcGG	Exon	HBB
	3	77	33c	tcaGCCCCACAGGGCAGTAAcGG	Intergenic	GRIN3A
R-03	1	70	55	cACGTTCACCTTGCCCCACAgGG	Exon	HBB
	2	62	58	cACGTTCACtTTGCCCCACAgGG	Exon	HBD
R-04	1	47	53	cCACGTTCACCTTGCCCCACaGG	Exon	HBB
	2	10	12	cCACGTTCACtTTGCCCCACaGG	Exon	HBD
R-05	1		51	aGTCTGCCGTTACTGCCCTGnGG	Exon	HBB
R-06	1		59	cGTTACTGCCCTGTGGGGCAnGG	Exon	HBB
R-07	1	68	61	aAGGTGAACGTGGATGAAGTtGG	Exon	HBB
	2		7	aAGGTGAACGTGGATGcAGTtGG	Exon	HBD
R-08	1	38	36	cCTGTGGGGCAAGGTGAACGtGG	Exon	HBB
	2		48	cCTGTGGGGCAAaGTGAACGtGG	Exon	HBD
R-25	0	50	46	GTGTTCATCTTTGGTTTTGTgGG	Exon	CCR5
	0	32	20	GTGTTCATCTTTGGTTTTGTgGG	Exon	CCR2
R-26	0	76	74	GCTGCCGCCCAGTGGGACTTtGG	Exon	CCR5
R-27	0	78	77	GGCAGCATAGTGAGCCCAGAgGG	Exon	CCR5
R-29	0		65	GTGAGTAGAGCGGAGGCAGGnGG	Exon	CCR5
R-30	0		21	GTAGAGCGGAGGCAGGAGGCgGG	Exon	CCR5
	2		5	GTAGAGCGGAGGCAGGAGttgGG	Exon	CCR2

^aNumber of base differences between the guide strand and complementary sequence, including the 5′ nucleotide.

^bBase pair positions from the PAM are numbered above the loci. The differences between the guide strand and complementary sequences are indicated in lowercase underlined nucleotides. The first of the three nucleotides in the PAM sequence is also indicated in lowercase.

^cT7E1 was performed in duplicate for this off-target site, not triplicate as with all other cases.