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. 2013 Oct 31;9(10):e1003731. doi: 10.1371/journal.ppat.1003731

Figure 4. Effects of TbKHC1 on T. brucei growth and infection in mice.

Figure 4

(A,B) In vitro parasite growth (A: monomorphic trypanosomes, either WT or induced and non-induced TbKHC1 KD; B: pleomorphic trypanosomes, either WT or TbKHC1 KO and rescued TbKHC1 KO). Data are means ± SEM of one representative from 3 independent experiments (C,D) Parasitemia in C57Bl/6 mice infected with pleomorphic WT and TbKHC1 KO parasites (C: intraperitoneal injection; D: injection through the bite of infected tsetse flies). Data are means ± SEM of 4 individual mice of one representative from 6 independent experiments. * p<0.05 comparing TbKHC1 KO- and WT-infected mice. (E–G) TbKHC1-mediated myeloid cell activation. At day 6 p.i. with WT and TbKHC1 KO parasites, mice were treated with anti-IL-10R (anti-IL-10R Ab) or control antibody (Ctrl Ab), and at day 7 spleen myeloid cells were analyzed for arginase activity (E), spontaneous NO secretion (F) and IL-10 secretion (G). Data are means ± SEM of 3 individual mice of one representative from 3 independent experiments. ∇ p<0.05 comparing WT- or TbKHC1 KO-infected to non-infected mice; £ p<0.05 comparing WT- or TbKHC1 KO-infected mice treated with anti-IL-10R antibody to WT- or TbKHC1 KO-infected control antibody-treated mice; # p<0.05 comparing TbKHC1 KO- and WT-infected mice treated with control antibody.