Figure 9.

Up-regulation of IL-23 in the liver after bile acid feeding. Mice were given either an AIN-93M diet supplemented with 0.3% cholic or an AIN-93M diet alone. A: IL-23 mRNA was measured in the liver by real-time PCR. Data are given as means ± SEM. IHC staining was used to detect IL-23 in sections of liver from mice fed an AIN-93M control diet (B) or an AIN-93M diet supplemented with 0.3% cholic acid (C). Positive staining for IL-23 appears red in the photomicrographs. The thick arrows indicate IL-23 staining in hepatocytes. The thin arrow indicates the area of IL-23 staining in the sinusoid. Scale bars: 50 μm. DAPI, which appears blue in the photomicrographs, was used to stain nuclei.