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. 2012 Sep 6;10:73. doi: 10.1186/1477-7827-10-73

Table 2.

Effects of cryoprotectant exposure, vitrification and time in the warming solution on cleavage of bovine oocytes and subsequent embryo development (Experiment 2)

Group GV oocytes (n) Cleavage rate n (%) Blastocyst rate n (% of total)
Control (non-exposed)
81
61 (75.3%)a
22 (27.2%)a
VS1
 
 
 
 1 min warming interval
82
60 (73.2%)a
16 (19.5%)a
 5 min warming interval
77
48 (62.3%)a
15 (19.5%)a
VS1 + VS2
 
 
 
 1 min warming interval
87
52 (59.8%)a
16 (18.4%)a
 5 min warming interval
82
54 (65.9%)a
22 (26.8%)a
Vitrified
 
 
 
 1 min warming interval
74
22 (29.7%)b
1 (1.4%)b
 5 min warming interval
98
21 (21.4%)b
2 (2.0%)b
P-value
 
 
 
Cryoprotectant exposure
 
0.202
0.205
Warming time
 
0.241
0.689
Cryoprotectant exposure * warming time   0.381 0.687

Values with different superscripts (a,b) in the same column differ (P < 0.05).

VS1: Vitrification solution 1.

VS2: Vitrification solution 2.