Table 2.
Effects of cryoprotectant exposure, vitrification and time in the warming solution on cleavage of bovine oocytes and subsequent embryo development (Experiment 2)
| Group | GV oocytes (n) | Cleavage rate n (%) | Blastocyst rate n (% of total) |
|---|---|---|---|
|
Control (non-exposed) |
81 |
61 (75.3%)a |
22 (27.2%)a |
|
VS1 |
|
|
|
|
1 min warming interval |
82 |
60 (73.2%)a |
16 (19.5%)a |
|
5 min warming interval |
77 |
48 (62.3%)a |
15 (19.5%)a |
|
VS1 + VS2 |
|
|
|
|
1 min warming interval |
87 |
52 (59.8%)a |
16 (18.4%)a |
|
5 min warming interval |
82 |
54 (65.9%)a |
22 (26.8%)a |
|
Vitrified |
|
|
|
|
1 min warming interval |
74 |
22 (29.7%)b |
1 (1.4%)b |
|
5 min warming interval |
98 |
21 (21.4%)b |
2 (2.0%)b |
|
P-value |
|
|
|
| Cryoprotectant exposure |
|
0.202 |
0.205 |
| Warming time |
|
0.241 |
0.689 |
| Cryoprotectant exposure * warming time | 0.381 | 0.687 |
Values with different superscripts (a,b) in the same column differ (P < 0.05).
VS1: Vitrification solution 1.
VS2: Vitrification solution 2.