Figure 4.

NEP inhibits the formation of p85-FAK protein complexes. (a) 500 μg of total cell lysates were immunoprecipitated with anti-p85 Ab, separated by SDS-PAGE, transferred to nitrocellulose, and Western blotted with anti-FAK antibody C-20 or anti-p85 antibody. (b) WT-5, TN-12, and M-22 cells were cultured with (+) and without (–) 1 μg tetracycline (with the addition of 100 nM CGS24592 in lanes 2 and 4). Cells were lysed and analyzed as described in a.