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. 2013 Oct 31;9(10):e1003753. doi: 10.1371/journal.ppat.1003753

Figure 4. BoMac myeloid cells express relatively less gp180 glycoprotein in comparison with epithelial cells.

Figure 4

A–B. MDBK and BoMac cells were infected with the BoHV-4 V.test strain at a MOI of 1. Twenty-four hours p.i., relative expressions of Bo10 spliced vs unspliced transcripts (A) and of Bo10 spliced vs ORF8 transcripts (B) were estimated as described in the Methods. The data presented are the average ± SEMs for 3 measurements and were analyzed by Student's t-test, ** p<0.01. C. Detection of the gp180, encoded by the Bo10 spliced transcript, and gB glycoproteins in bovine epithelial and myeloid cells. MDBK and BoMac cells were infected with the BoHV-4 V.test strain at a MOI of 1. Twenty-four hours p.i., these cells were subjected to western blotting with anti-Bo10-c15 serum (recognizing gp180) and mAb 35 (recognizing gB) as described in the Methods. On the gp180 blot, open and filled triangles indicate respectively the specific 180 kDa protein and a background band. On the gB blot, open and filled arrow heads indicate uncleaved gB and furin-cleaved gB C-terminus respectively. The position of a molecular mass (MM) standard (in kDa) is shown on the left.