Figure 2.

Axonal degeneration, 2 months after EAE induction. EAE mice were given a control diet or a diet containing resveratrol on days −1 to 8 (Early), days 14 to 23 (Late), or days −1 to 63 (Whole). A: Immunohistochemistry for nonphosphorylated neurofilament reveals higher levels of axonal damage (arrows) in the Early group than in the Control group. Boxed regions are shown at higher magnification in the corresponding inset. B: Higher numbers of nonphosphorylated neurofilament-positive damaged axons are detected in the spinal cord of mice fed a diet containing resveratrol during the induction phase of EAE, compared with control mice. Data are expressed as means ± SEM. n = 5 or 6 symptomatic mice per group. ^∗∗P < 0.01, ANOVA. Original magnification: ×83; ×115 (insets). Scale bars: 50 μm (A).