Figure 5.

Regulators of myostatin are increased in the hypertrophied golden retriever muscular dystrophy (GRMD) cranial sartorius (CS) muscle. A: cfa-miR-208b_st was increased in GRMD (D) CS compared to normal (N) CS (fold change = +2). B: cfa-miR-539_st was increased in GRMD CS compared to normal CS (fold change = +3). C: AKT1 phosphorylation at serine 473 was increased in GRMD and positively correlated with CS circumference at 6 months (r = +0.78; r² = 0.6; P < 0.05; n = 8). miRNA profiling was used to detect target miRNAs. Western blot and densitometry was used to detect AKT1 phosphorylation status. Numbers to the left of the membranes and gels indicate molecular weight (kDa). MHC, myosin heavy chain. ^∗P < 0.05