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. 2013 Nov;183(5):1411–1424. doi: 10.1016/j.ajpath.2013.07.013

Figure 8.

Figure 8

Immunofluorescence microscopy of α-dystroglycan (DAG1), β-sarcoglycan (SGCB), and β-spectrin (SPTBN1) reveals variable expression in golden retriever muscular dystrophy (GRMD) muscles at 6 months. A: In normal muscle, DAG1 is expressed at the membrane in every myofiber observed. B and C: DAG1 is variably expressed at the membrane in GRMD cranial sartorius (CS; B), long digital extensor (LDE), and vastus lateralis (VL) muscles (C), but appears to have greater intensity in the CS. D: SGCB is expressed at the membrane of every myofiber in normal dogs. E and F: In GRMD dogs, SGCB is expressed at the membrane of myofibers in the CS (E), LDE, and VL (F), but less than normal samples. No differences in intensity are seen among the three GRMD muscles. G: SPTBN1 is expressed at the myofiber membrane in all normal muscles. H and I: The intensity of expression in the GRMD CS (H) is greater than normal and other affected muscles (I). J: Myotrophin (MTPN) is expressed in the perimembranous region of myofibers in normal muscle. K: GRMD CS muscle shows perimembranous staining. L: GRMD VL samples at 6 months showed slightly increased perimembranous myofiber MTPN. Original magnification, ×100 (A–L). Scale bars: 50 μm (A–L).