Figure 2.

ARTD1 methylation by SET7/9 does not influence its PARylation or acetylation but is inhibited by ARTD1 auto-modification. (a) Recombinant ARTD1 was methylated by SET7/9 after ADP-ribosylation in the presence or absence of DNA and 3-AB. ±: 3-AB was added after the ADP-ribosylation reaction. (b) ARTD1 was first incubated with SET7/9 in the presence or absence of ¹⁴C-SAM and afterwards incubated with activating DNA and cold NAD⁺ to allow auto-modification. (c) ARTD1 (373–524) was first incubated with WT SET7/9 or an enzymatic dead mutant (H297A) in the presence of cold SAM and afterwards acetylated with p300 and ¹⁴C-AcCoA (i). Methylation was controlled with ¹⁴C-SAM (ii). All experiments were repeated at least twice, gave a similar result, and one representative blot is shown.