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. 2013 Oct;3(10):120173. doi: 10.1098/rsob.120173

Figure 4.

Figure 4.

Methylation-deficient ARTD1 is less active and less efficiently recruited to sites of local DNA damage induced by femtosecond laser irradiation. (a) ARTD1 knockout MLFs were stably complemented with WT ARTD1 or two methylation-deficient mutants. Cells were then fractionated and CEs and NEs were analysed by western blot. (b) ARTD1 activity in NE from (a) was analysed by radioactive PAR assays in the absence or presence of 5 pmol-activating DNA. Experiments in A and B were repeated twice with a similar outcome and one representative blot is shown. Quantifications are shown in the electronic supplementary material, figure S5a,b. (c) Recruitment of WT and K508R ARTD1 to sites of local DNA damage induced by femtosecond laser irradiation at λ = 1050 nm. (d) Recruitment of macroH2A1.1-EGFP to sites of local DNA damage induced by femtosecond laser irradiation at λ = 1050 nm. (e) Recruitment of WT and K508R ARTD1 to sites of DNA damage by femtosecond laser irradiation at λ = 775 nm.