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1H,15N (TROSY) HSQC spectra of protein constructs from this study. A, 1H,15N-HSQC NMR spectra of 2H,15N-apo-HisJ (red), 15N-D1 (blue), and 15N-D2 (green) protein constructs. Residues from the inserted flexible linker region of D1 are labeled. B, 1H,15N TROSY-HSQC spectra of 2H,15N-apo-HisJ (red) and 2H,15N-holo-HisJ (black).
