FIGURE 11.
SAP is required for progression of collagen-induced arthritis. A, this experiment was performed as detailed in Fig. 10, except that TAM treatment was initiated 29 days after immunization. B, anti-CII antibodies were measured 10 days (D10) after immunization of wild-type mice with CII when the clinical symptoms were not yet observed. C, high-affinity IgG2a antibodies against CII were measured on day 14 after CII injection using the chaotropic agent NaSCN. D, high-affinity IgG2a and IgG2b antibodies against CII were measured on the indicated days after CII injection. Induction of RA (E and F) and anti-CII antibodies (G) was monitored as detailed in Fig. 1. Time of TAM treatment is shown by a red arrow. Values for individual mice are depicted by individual symbols, and the horizontal line shows mean values. p values are also shown. H, the abundance of high-affinity anti-CII IgG2a was determined by performing ELISA in presence of progressively higher concentrations of NaSCN. CTL, control mice; iKO, inducible KO mice; CFA, complete Freund adjuvant.