FIGURE 2.

Membranes of A. woodii catalyze ferredoxin-dependent reduction of NAD⁺ with CO + Acs/CODH as reducing agent. Anaerobic cuvettes in an atmosphere of 0.5 × 10⁵ Pa of CO contained 1 ml buffer containing 50 mm Tris-HCl, 20 mm NaCl (pH 7.7), 2 mm DTE, and 2 μm resazurin prepared in 3-fold distilled water. The addition of 30 μg of Acs/CODH, 4 mm NAD⁺, A. woodii membranes, and 30 μm ferredoxin led to an increase in absorption at 340 nm.