FIGURE 4.

SN inhibits RAD54 ATPase but does not significantly disrupt RAD54-DNA complexes. a, the effect of 0–50 μm SN on 20 nm RAD54 ATPase in the presence of PX junctions (oligos 169/71/170/171) (1 nm, molecules). Hydrolysis of ATP was monitored using a spectrophotometric assay. b, the effect of SN (0–50 μm) on the formation of 100 nm RAD54 complexes with PX junctions (33 nm, molecules) was analyzed using electrophoresis in a 4% polyacrylamide gel. c, the data from b presented as a graph. Error bars represent the S.E.