Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 10;288(44):31581–31591. doi: 10.1074/jbc.M113.514471

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 6. — Self-association of Lcaf protein. A, elution profiles of the h:Lcaf complex (top) and h:Lcaf protein (bottom) in gel filtration chromatography. Numbers at the top indicate fraction numbers of the chromatography. Elution of markers is shown on the horizontal axis. M and IN at the top are the molecular mass marker and input protein fraction on chromatography, respectively. B, self-association of Lcaf in yeast two-hybrid analysis. HIS3 gene was used as a reporter gene. Top, schematic structures of Lcaf hybrid proteins. LUFS, the conserved domain, named after the four founding members LUG, LUH (for LEUNIG_HOMOLOG), yeast Flo8, and human SSDP. Bottom, each yeast transformant was inoculated on an SD agar plate containing 10 mm 3-AT without histidine or on SD agar with histidine.