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Idas is the preferred dimerization partner for Geminin. U2OS cells were (co)transfected with full-length Geminin-GFP, Geminin-HA, and/Idas-GFP or GFP as indicated. Cells transfected with GemininGFP alone served as the negative control. Total cell lysates (left) and anti-HA immunoprecipitates (IP, right) were immunoblotted for GFP (upper) and HA (lower). When both Idas and Geminin were available in similar amounts, Geminin preferred to heterodimerize with Idas.
