FIGURE 1.

IbpB preferentially functions under heat shock conditions. A, immunoblotting results of endogenous IbpB in wild-type cells cultured under the indicated stress conditions using the anti-IbpB polyclonal antibody. TnaA was used as a loading control. B, immunoblotting results of in vivo photocross-linked products of Tyr-45 Bpa expressed in ΔibpB cells using the anti-His tag antibody. Cells were grown at 30 °C and then subjected to stress treatment for half an hour by heating at 50 °C or by adding to a final concentration of 5 mm H₂O₂ or 10% ethanol before further analysis.