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Kinetic analysis of hydroxylated jasmonate accumulation in wild-type (WT), cyp94b3, and cyp94b3c1 mutant plants upon leaf wounding. Leaves were harvested at increasing times after wounding and extracted for jasmonate determination by UPLC-MS/MS. A, JA; B, 12OH-JA; C, 12OH-JA-Ile. Data are mean ± S.E. from three biological samples. Asterisks indicate significant differences between mutant and WT at p < 0.05 (*) or p < 0.001 (***) (two-way ANOVA).
