FIGURE 6.

Kinetic analysis of jasmonate accumulation in wild-type (WT) and in iar3 and ill6 mutant plants upon leaf wounding. iar3-5 with ill6-1 and iar3-6 with ill6-2 lines were analyzed along with WT plants in independent experiments. Leaves were harvested at increasing times after wounding and extracted for jasmonate determination by UPLC-MS/MS. A, JA-Ile; B, JA; C, 12OH-JA-Ile; D, 12OH-JA; E, 12COOH-JA-Ile. Data are mean ± S.E. from three biological samples. Asterisks indicate significant differences between mutant and WT at p < 0.05 (*), p < 0.01 (**) or p < 0.001 (***) (two-way ANOVA).