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. 2013 Sep 13;288(44):31738–31751. doi: 10.1074/jbc.M113.479535

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — UV/visible spectral properties of full-length Agp2 at different pH values. A, spectra of dark-adapted form (Pfr). B, spectra after far-red irradiation showing a mixture of Pr and Pfr. C, calculated Pr spectra. D, absorption of Pfr and Pr at their respective maximum at different pH values. The Henderson-Hasselbalch (HH) fit to the Pr data yielded a pK_a of 7.59. E, estimated fraction (f = Pfr/Ptot) of far-red-irradiated samples as shown in B. F, difference spectra of far-red-irradiated minus dark-adapted Agp2. G, absorbance change ratios from these difference spectra.