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. 2013 Sep 13;288(44):31738–31751. doi: 10.1074/jbc.M113.479535

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Flash photolysis measurements of the Pr to Pfr photoconversion of Agp2. Left, transient absorption difference spectra of pre-illuminated phytochrome (785 nm) after flash irradiation of Pr (660 nm) at pH 6, 7.8, and 9 at selected times as indicated. Middle, related decay-associated spectra A_i. Right, same as middle but sum of A₀ and A₄ instead of A₀. The thin arrows indicate the time course of signal amplitude. The gray rectangle marks the region of pump pulse artifacts. a.u., absorbance units; mOD, milli-OD units.