FIGURE 4.

The exosome cofactors Rrp47p and Mpp6p contribute to the targeting and elimination of Rho-induced aberrant mRNPs upon cotranscriptional recruitment. A, the wild-type strain and single deletion mutants rrp47Δ and mpp6Δ were transformed with the plasmid expressing Rho. Strains grown selectively under repressing conditions were spotted for growth as described in Fig. 2A. B, Northern blot analysis was made as in Fig. 2B. C, semiquantitative RT-PCR analysis and RT-qPCR quantifications to measure the level of PMA1 mRNA were done as in Fig. 2C. D, quantification of the fold enrichment for PMA1 DNA (5′, middle, and 3′ regions) in immunoprecipitates from cells harboring Myc-tagged Rrp47p or TAP-tagged Mpp6p. Immunoprecipitated samples (output) were normalized to input as in Fig. 2D. E, Western blot analysis of whole protein extracts isolated from cells harboring Myc-tagged Rrp47p or TAP-tagged Mpp6p was conducted as in Fig. 1C.