FIGURE 7.

The removal of Rho-induced aberrant mRNPs requires TRAMP component Air2p but not Air1p. A, the wild-type strain, single deletion mutants air1Δ and air2Δ, as well as a double mutant, air1Δair2Δ, were transformed with the Rho-expressing plasmid. Strains grown selectively under repressing conditions were spotted for growth as described in Fig. 2A. B, Northern blot analysis was made as in Fig. 2B. C, semiquantitative RT-PCR analyses and PMA1 mRNA level determinations by RT-qPCR were performed as in Fig. 2C. D, Western blot analysis to evaluate the presence of Rrp6p in the different strains was performed as in Fig. 5A. E, ChIP analyses of the recruitment of TAP-tagged Air1p or Air2p to the PMA1 gene were performed as in Fig. 2D. F, Western blot analysis to evaluate the level of Air1p and Air2p in the strains analyzed by ChIP. PAP, peroxidase-anti-peroxidase. G, comparison of the recruitment to the PMA1 gene of Myc-tagged Trf4p and Trf5p in wild-type or air2Δ backgrounds. The ChIP analyses were performed as in Fig. 2D. H, evaluation of Trf4p and Trf5p levels in the strains analyzed by ChIP.