TABLE 1.

Estimates of the % helical contents and melting temperatures (T_m) for each 11H and 15H peptide and associated mutant

Melting curves were only performed once. T_m was estimated as described under “Experimental Procedures.”

Peptide	Mutation/WT	α-Helical content, pH 7.4, (%) ± S.E.	Tm pH 7.0 (10 °C)	ΔTm compared to WT
11H1	WT	79 ± 1	26.9
11H1	N1327K	66 ± 2	22.3	−4.6
11H1	E1356K	62 ± 2	26.2	−0.7
15H1	WT	91 ± 1	29.7
15H1	N1327K	81 ± 3	27.8	−1.9
15H1	E1356K	90 ± 2	30.0	+0.3
15H1	R1382W	98 ± 2	33.8	+4.1