TABLE 1.
Peptide | Mutation/WT | α-Helical content, pH 7.4, (%) ± S.E. | Tm pH 7.0 (10 °C) | ΔTm compared to WT |
---|---|---|---|---|
11H1 | WT | 79 ± 1 | 26.9 | |
11H1 | N1327K | 66 ± 2 | 22.3 | −4.6 |
11H1 | E1356K | 62 ± 2 | 26.2 | −0.7 |
15H1 | WT | 91 ± 1 | 29.7 | |
15H1 | N1327K | 81 ± 3 | 27.8 | −1.9 |
15H1 | E1356K | 90 ± 2 | 30.0 | +0.3 |
15H1 | R1382W | 98 ± 2 | 33.8 | +4.1 |