FIG. 5.

IFN and CD8⁺ cell fluids with anti-HIV activity elute in similar high-performance liquid chromatography (HPLC) fractions. A CD8⁺ cell fluid having anti-HIV activity and commercial HLI (1,000 U/mL of F12 medium) were fractionated by HPLC using an ion-exchange column. Shown are the chromatograms for each sample. The various fractions were coanalyzed for the ability to suppress HIV replication in primary CD4⁺ cells and for IFN-α concentration by ELISA. The results are shown below the chromatograms. NA denotes fractions that were not evaluated for anti-HIV activity. Results are representative of 2 separate experiments.