Figure 1.

Schematic overview of the in silico nanodissection workflow, an iterative approach for cell-lineage–specific gene prediction, validation, and functional analysis. Expert-curated literature annotations are iteratively combined with gene-expression data to predict genes specific to a cell lineage. These predictions are assessed, and the standards are refined. Validation of podocyte specificity of our predictions used publicly available resources followed by evaluation of intrarenal mRNA and protein expression analysis in correlation with clinical phenotypes to define regulation of predicted gene sets in human disease.