Fig. 2.

Ang II stimulated NADPH-dependent superoxide production is modulated by mitochondrial ETC inhibitors in hVSMC. Cells were pre-incubated with Ang II (1×10⁻⁷ mol/L) for 1 h then lysates were used for the measurement of NADPH-dependent superoxide production using lucigenin chemiluminescence. NADPH stimulated ${\mathrm{O}}_2^{-}$ production was derived from the relative light units (RLU)/minute/100 μg of protein over a 40-min period. A, complex I inhibitor, rotenone significantly reduced Ang II-induced ${\mathrm{O}}_2^{-}$ production. Bars represent mean+SD, n=3–4. ^⁎⁎p<0.01 compared with control and ^††p<0.01 compared with Ang II. B, Complex II inhibitor TTFA, did not affect Ang II-induced ${\mathrm{O}}_2^{-}$ production. Bars represent mean+SD, n=2–4. ^⁎p<0.05 compared with control.