Fig. 5.

High oxygen conditions (21%) induce elevated levels of protein aggregates in old MPCs. Protein aggregates were measured in the cytosolic fraction isolated from MPCs with and without oxidative treatment (100 μM H₂O₂ for 19 h). Young MPCs are represented by black bars for 3% O₂ and hatched bars for 21% O₂. Old MPCs are represented by white bars for 3% O₂ and gray bars for 21% O₂. Each point represents the mean±SE of three independent experiments. Statistical significance with respect to untreated cells, ^⁎p<0.05.