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Modulating allergenicity of Bet v 1 through carbohydrate coupling. a) IgE-sensitized rat basophil leukemia (RBL) cells were stimulated with increasing concentrations of Bet v 1 coupled to increasing amounts of mannan (mannan:protein w/w ratios of 0.4–2.2). b) Allergenic potential in terms of the IgE cross-linking capacity compared to the wild-type protein, based on the 50%-release log-phase of the Gaussian release curve (EC50 value).
