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Predicted interaction between C. cinereus wild-type Nbs1 and Nbs1-2 protein with the phosphorylated threonine of Ctp1. S. pombe Nbs1 and C. cinereus Nbs1 were aligned using ClustalW and the nbs1-2 mutation was mapped onto the S. pombe Nbs1 crystal structure. The phenylalanine likely occludes the binding pocked for Ctp1.
