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. 2013 Nov 1;8(11):e79910. doi: 10.1371/journal.pone.0079910

Figure 3. The first and second zinc fingers are sufficient for nuclear localization.

Figure 3

(A) The ZF1-2- GFP construct is a fusion of the first and second zinc fingers to GFP. ZF1-GFP is a fusion of the first zinc finger to GFP. ZF2-GFP is a fusion of the second zinc finger to GFP. HEK293 cells were transfected with the indicated constructs, and direct immunofluorescence was conducted as described in Figure 1. (B) The subcellular localization of each mutant is summarized on the right. Representative cells from each construct are shown. (C) Results (mean±S.E.M., n=100) for quantitative analysis of images to determine the nuclear to cytoplasmic fluorescence ratio (Fn/c).