Table 1. Summary of MDCK-mediated mutations for each of the affected influenza A/H3N2 virus isolates examined in this study.
| Strain ID | Nucleotide changea | Amino acid change |
|---|---|---|
| Segment 1/ polymerase basic 2 (PB2) | ||
| C2011.422 | 2118A>R | I697M |
| C2011.496 | 600A>M | E191D |
| C2011.647 | 1287C>T | F420c |
| 1362T>C | L445c | |
| H2009.518 | 1724A>T | Q566L |
| H2010.619 | 1589Y>C | I/T521T |
| H2010.822 | 2200C>Y | L725c |
| H2011.507 | 2013T>W | T662c |
| H2011.797 | 1836G>K | M603I |
| Segment 4/ hemagglutinin (HA) | ||
| C2009.485a | 572C>M | N165N/Kb |
| C2011.301 | 739C>Y | P221P/Lb |
| C2011.564 | 489G>K | A138A/Sb |
| C2011.614 | 739C>Y | P221P/Lb |
| H2009.679 | 729G>R | G218G/Rb |
| H2010.559C | 739C>Y | P221P/Lb |
| 744G>R | V223V/Ib | |
| H2011.797 | 729G>R | G218G/Rb |
| H2011.808bC | 644G>R | K189 b , c |
| Segment 5/ nucleoprotein (NP) | ||
| C2011.471 | 123G>R | R26c |
| H2010.822 | 1262C>Y | T406T/I |
| H2011.570 | 1002C>Y | N319c |
| Segment 6/ neuraminidase (NA) | ||
| C2009.863 | 268A>R | E83c |
| 470G>R | D151D/N | |
| C2010.937V | 121A>R | T34c |
| 471A>R | D151D/G | |
| C2011.301 | 470G>R | D151D/N |
| C2011.362V | 470G>R | D151D/N |
| C2011.452 | 470G>R | D151D/N |
| C2011.471 | 470G>R | D151D/N |
| C2011.477 | 470G>R | D151D/N |
| C2011.493 | 471A>R | D151D/G |
| C2011.496 | 470G>R | D151D/N |
| C2011.507V | 470G>R | D151D/N |
| C2011.573 | 471A>R | D151D/G |
| C2011.614 | 471A>R | D151D/G |
| C2011.641 | 471A>R | D151D/G |
| C2011.825 | 470G>R | D151D/N |
| H2010.321C | 470G>A | D151N |
| H2010.559C | 251T>Y | C78C/R |
| H2010.797 | 471A>R | D151D/G |
| H2011.463 | 1066G>R | V349c |
| H2011.482 | 470G>R | D151D/N |
| H2011.507 | 395C>Y | P126P/S |
| H2011.570 | 471A>R | D151D/G |
| Segment 8/ non-structural (NS) | ||
| C2011.362V | 592R>G | NS1: D/G189G; NS2: I/V32V |
| C2011.573 | 578R>A | NS2: G/D27D |
| H2010.559C | 335C>Y | NS1: F103c |
Alternatively spliced mRNAs of the non-structural protein gene allows translation of two alternate proteins, non-structural protein 1 (NS1) and non-structural/ nuclear export protein (NS2/NEP). The non-ATCG letters coded for ambiguous/degenerate nucleotide sequences, i.e. “R” - “A and G”, “M” – “A and C”, “Y” – “C and T”, “W” – “A and T”, and “K” – “G and T”.
aNucleotide numbering based on influenza A/Singapore/C2009.458V/2009(H3N2), GenBank accession: KF015129, KF014198, KF014597, KF014464, and KF014730.
bAmino acid numbering without including signaling peptide (i.e. the first 16 amino acids)
cChange of nucleotide or amino acid that resulted in synonymous mutation.