Figure 5.

Support of expansion of healthy clonogenic progenitors by MDS-MSC is defective and modified by LEN. MSC were seeded on 35 mm plates and used for co-culture with 1×10³ healthy CD34⁺ cells using semi-solid media. After 14 days of co-culture, hematopoietic colony output was determined under an inverted microscope; the figure shows differences of colony type and numbers of each studied group and compares them to the baseline CFU-GEMM potential of the HSPC on an assay without stroma present. Results are expressed as mean ± SEM of independent cases. Numbers were HSPC alone n=3, Healthy n=5, LR-5q n=6, LR n=5 and HR n=5. Significance was set as * P≤0.05; **P≤0.005; *** P≤0.001.