Table 1.

Examples of single‐cell genome sequencing.

Microorganism	Assembled bases (Mb)	Estimated % genome recovery	Scaffolds	Contigs	GC%	Single cell separation	Isolation source	Reference
TM7a (new phylum)	2.865	?	1825		34.3	Microfluidics	Human mouth biofilm	Marcy et al. (2007b)
TM7_GTL1 (new phylum)	0.679	?		132	48.5	FISH/FACS	Soil	Podar et al. (2007)
Prochlorococcus MED4		95		755		FACS	Sea water; lab culturea	Rodrigue et al. (2009)
Flavobacterium MS024‐2A	1.905	91		17	36	Flow cytometer	Coastal water, Maine, USA	Woyke et al. (2009)
Flavobacterium MS024‐3C	1.505	78		21	39	Flow cytometer	Coastal water, Maine, USA	Woyke et al. (2009)
Cand. Sulcia muelleri DMIN	0.244	100	1	1	22.5	Micromanipulator	Symbiont from insect bacteriome (green sharpshooter)	Woyke et al. (2010)
Poribacteria	1.885	66		1597	53.4	FACS	Symbiont from marine sponge	Siegl et al. (2011)
Cand. Nitrosoarchaeum limnia SFB1	1.690b	95	26	136	32.4	Microfluidics, laser tweezer	Ammonia‐oxidizing enrichment culture; sediment water, San Fransisco bay, USA	Blainey et al. (2011)

^a.Method validation using strain with known genome sequence.

^b.Pooled sequence data from five individual cells; see Table 2.