Thermoanaerobacterium saccharolyticum JW/SL‐YS485 |
Department of Energy (DOE) Joint Genome Institute |
Autoplast generation with cell wall‐destabilizing agent (niacin), subsequent sucrose‐containing buffer washes |
Electroporation (12.5 kV cm−1, 400 ohm, 25 µF) |
Approximately 103–4
|
Tyurin et al. (2006), Shaw et al. (2008) |
Thermoanaerobacter ethanolicus JW200 |
DOE Joint Genome Institute |
Glycine‐ and sucrose‐induced protoplast formation, subsequent glycerol‐containing buffer washes |
Electroporation (13 kV cm−1, 400 ohm, 25 µF) |
Approximately 101
|
Peng et al. (2006) |
Thermoanaerobacter mathranii BG1 |
Not recorded |
Cellobiose wash buffer. Isoniacin addition |
Custom built electroporator with custom cuvettes (2mm gap). 10 ms square wave pulse. 25 kV cm−1
|
102−5
|
Yao and Mikkelsen (2010) |
Geobacillus thermoglucosidasius NCIMB 11955 |
Not recorded |
High osmolarity washing buffer (sorbitol and mannitol) |
Electroporation (25 kV cm−1, 600 ohm, 10 µF) |
104
|
Taylor et al. (2008), Cripps et al. (2009) |
Geobacillus thermoglucosidasius DL33 |
Not recorded |
High osmolarity washing buffer (sorbitol and mannitol) |
Electroporation (25 kV cm−1, 600 ohm, 10 µF) |
103−4
|
Taylor et al. (2008), Cripps et al. (2009) |
Clostridium thermocellum |
DOE Joint Genome Institute |
Isoniacin addition |
Custom built electroporator with custom cuvettes (2mm gap). 10 ms square wave pulse. 25 kV cm−1
|
103
|
Tyurin et al. (2004) |
Rhodothermus marinus (trpB‐) |
DOE Joint Genome Institute |
Glycerol treatment |
Electroporation (22.5 kV cm−1, 200 ohm, 25 µF) |
106
|
Bjornsdottir et al (2005; 2006; 2007) |
Thermosynechococcus elongatus BP‐1 |
Kasuza DNA Research Institute |
2mM N‐tris(hydroxymethyl)methyl‐2‐aminoethanesulfonic acid (TES) treatment |
Electroporation (10 kV cm−1, 200 ohm, 25 µF) and natural competency |
109
|
Iwai et al. (2004) |
Thermotoga maritima, T. neapolitana |
J. Craig Venter Institute |
Lysozyme treatment and DNA encapsulation with liposome reagent |
Spheroplast creation and cationic liposome integration |
102
|
Noll and Vargas (1997), Yu et al. (2001), Conners et al. (2006) |
Thermus thermophilus HB27 |
Goettingen Genomics Laboratory |
Glycerol treatment |
Natural competency and electroporation (25 kV cm−1) |
109
|
Friedrich et al. (2001) |
Pyrococcus furiosus DSM 3638 |
Universities of Utah and Maryland |
CaCl2 treatment |
Heat shock at 80°C |
102
|
Waege et al. (2010) |
Thermococcus kodakaraensis KOD1 |
Kyoto University |
CaCl2 treatment |
Heat shock at 85°C |
102
|
Fukui et al. (2005), Sato et al. (2005) |
Sulfolobus islandicus, S. solfataricus |
University of Copenhagen, DOE Joint Genome Institute |
Sucrose treatment in growth medium and wash buffers |
Electroporation (7.5 kV cm−1, 400 ohm, 25 µF) |
103
|
She et al. (2001; 2009), Albers and Driessen (2008) |