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. 2011 Jul 6;4(4):523–532. doi: 10.1111/j.1751-7915.2011.00253.x

Figure 3.

Figure 3

Evaluation of antifungal metabolites produced by B. subtilis HC8 and B. amyloliquefaciens FZB42. A. TLC analysis of methanol extract of the supernatant fluids of Bacillus strains. The plate was developed in chloroform/methanol/water 65:25:4 (v/v/v) for 2.5 h. For visualization, the developed plate was stained in iodine followed by dipping in 1% aqueous starch. Pure iturin A (It) was used as a reference. HC8, endophytic strain B. subtilis HC8; FZB42, Bacillus amyloliquefaciens FZB42; ΔbmyA, mutant of FZB42 unable to produce bacillomycin D; ΔfenA, mutant of FZB42 unable to produce fengycin; t‐z, major spots of the HC8 crude extract; z, likely correspond to iturin; s, fraction likely to contain bacillomycin D; w and t likely to contain fengycin. B. Antifungal activity of individual fractions of crude extract from B. subtilis HC8 towards Forl in vitro. t‐z, major fractions corresponding to spots in A).